Protein Labeling Reagents
Biotium MemBrite™ Fix 488/515 Cell Surface Staining Kit, 500 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This kit has reagents sufficient for 500 labeling reactions. The kit contains 5 vials of MemBrite™ Fix 488/515. Each vial is sufficient for 20 uL of 1000X dye after DMSO reconstitution.
Bachem 6-Carboxy-fluorescein
6-Carboxy-fluorescein, 3301-79-9, C21H12O7, Mr 376.32, 500mg. 6-FAM, 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-terephthalic acid. Widely used green-fluorescent labeling dye.
Proteintech Group Inc Histone-Label Atto488
Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei. It consists of an anti-histone VHH conjugated to the fluorescent dye ATTO488. For IF, microscopy of histones, chromosomes, and nuclei.
Aat Bioquest 6-TAMRA cadaverine
6-TAMRA cadaverine is a building block for developing fluoresceinated bioconjugates. It is also a fluorescent transglutaminase substrate to label proteins by transaminidation.
Aat Bioquest Fluorescein-5-maleimide *CAS 75350-46-8*
Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or resulted from reduction of cystines.
Aat Bioquest iFluor™ 488 amine
Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
Biotium CF660C, Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF660C dye has excitation/emission at 667/685 nm.
Biotium CF405M Protein Labeling Kit , 3x(1mg) labelings
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF dye succinimidyl ester (CF dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405M dye has excitation/emission at 408/452 nm.
Biotium LysoView™ 540, 1000X in DMSO, Trial Size
LysoView™ 540 is a red fluorescent dye that is highly selective for labeling and tracking of lysosomes in live cells, with no wash step required. With absorption and emission at 540/634 nm, the dye can be detected using a Cy3 filter set.
Biotium MitoView™ 720, 1000X in DMSO
MitoView™ 720 is a near-infrared live cell mitochondrial dye with absorbance/emission at 720/758 nm. While optimally detected using Cy7 settings, the dye is bright enough to be imaged in the Cy5 channel.
Biotium Tyramide Amplification Kit with HRP Streptavidin and CF488A Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP Streptavidin CF488A dye tyramide. The dye has an excitation/emission of 490/515 nm. The kit contains sufficient reagent for 50-150 slides.
Biotium Phalloidin, CF594, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 has excitation/emission at 593/614 nm.
Biotium Mix-n-Stain™ CF594 Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm. It is suitable for labeling ligands for cell surface staining.
Biotium Cyanine 647 Succinimidyl Ester
Biotium offers high quality amine-reactive succinimidyl ester (SE, or NHS-Ester) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy3), Cyanine 647 (Cy5), Cyanine 770 (Cy5.5), and Cyanine 750 (Cy7) as high quality succinimidyl esters. Also see our CF Dye Succinimidyl Ester, available with more than 20 bright and photostable CF Dyes. Cyanine 647 is structurally identical to Cy5 from GE Healthcare. Properties: Ex / Em (water) = 650/665 nm; Dark blue solid soluble in DMF and DMSO; Store at -20°C and protect from moisture and light; C 43 H 58 N 4 O 10 S 2; MW: 855. Cy3, Cy5, Cy5.5, and Cy7 are registered trademarks of GE Healthcare.